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The results of studying the concentration distribution of volatile components of exhaled air using chromatography, fluorescence spectroscopy, and film-sol-gel sensor structures are given. The creation and study of the properties of gas-sensing composite materials sensitive to molecules of acetone and ammonia of ultra-low concentration is described. Polymeric structures based on polyvinyl alcohol and chitosan are described, as well as the study of the conditions of interphase gas permeability and the processes of mass transfer of active components from them to the model medium. The processes of oxygen and carbon monoxide transport and their detection in blood by non-invasive methods of modulation optical spectroscopy of the near-infrared range were 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